The Annunciation is a Christian celebration of the iconic moment when the archangel Gabriel announced to the Virgin Mary that she would conceive and become the mother of the Son of God, Jesus. The story of the Annunciation derives primarily from the biblical Gospel of Luke and has been depicted abundantly and variously in many forms of visual art since the first centuries of Christianity and in Christian iconography. This essay will explore the depiction of the Annunciation and symbolism in the Italian Renaissance period and will pay particular attention to the development of attention from spiritualism towards naturalism through refined expressive and gestural language from the 14th century to the end of the 15th century. As a student of Duccio di Buoninsegna, who was an Italian artist and worked actively in the city of Siena, Tuscany, Simone Martini's most famous painting "The Annunciation and the Two Saints" (1333) (Fig. 1) shows the influence of the Sienese style of painting at a time when Byzantine art was very popular in the 14th century (Kleiner, 2010:387). This is made evident by the extensive use of gold in the background space behind the figures and flowing throughout the painting on panels, the attention paid in particular to the decorative pattern, as well as the detail and subtle use of sinuous lines and rich colors to create patterns. , which are all characteristics of Sienese-style religious iconography. The painting is considered very beautiful, executed with narrow, elegant brushstrokes and shows a medieval interest in depicting the supernatural and spiritual. The overall artwork is composed of a large panel containing "The Annunciation" with two others flanking it, said to have been painted by Lippo Memmi, who...... in the center of the sheet.... ..w York, NY. Britannica Educational Publishing.- Kulenovic, R. 2004. The Annunciation Lionardo da Vinci [online]. Available: http://www.johner.moreinfo.se/museer-firenze-uffizierna-annunciation-en.shtml [2014, May 14]- BibliographyBibliography- Shea, R. 2004. Marcia Myers: Twenty Years Paintings & Works on Paper 1982–2002. Manchester, United States: Hudson Hills.- The Bible: English Standard Version. 2001. Illinois, United States of America. Crossway Bibles.- Van Dijk, A. 1999. The angelic greeting in Byzantine and medieval Annunciation images. The Art Bulletin. 81(3): 420-436).- Von Rohr Scaff, S. 2002. The Virgin Annunciate in the Italian art of the Late Middle Ages and the Renaissance. University literature. 29(3):109-123.- Web Art Gallery. nd Available: http://www.wga.hu/frames-e.html?/html/l/leonardo/01/2annunc.html [2014, May 10]